Structural basis for misaminoacylation by mutant E. Perona JJ, Swanson RN, Rould MA, Steitz TA, Söll D.Transfer RNA mischarging mediated by a mutant Escherichia coli glutaminyl-tRNA synthetase. Inokuchi H, Hoben P, Yamao F, Ozeki H, Söll D.coli glutaminyl-tRNA synthetase complexed with tRNA(Gln) and ATP at 2.8 A resolution. Rould MA, Perona JJ, Söll D, Steitz TA.Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase. Sampson JR, DiRenzo AB, Behlen LS, Uhlenbeck OC.Normanly J, Ogden RC, Horvath SJ, Abelson J.Association of transfer RNA acceptor identity with a helical irregularity. McClain WH, Chen YM, Foss K, Schneider J.Parameters for the molecular recognition of transfer RNAs. Aminoacylation of RNA minihelices with alanine. Changing the identity of a tRNA by introducing a G-U wobble pair near the 3' acceptor end. A simple structural feature is a major determinant of the identity of a transfer RNA. Links to PubMed are also available for Selected References. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Full textįull text is available as a scanned copy of the original print version. It is concluded that both positive and negative discrimination toward the binding of tRNA anticodon sequences is restricted to a limited region of the synthetase, residues 451-467. Analysis of catalytic properties of purified suppressor enzymes shows that they have acquired an additional specificity toward the amber anticodon without complete disruption of the methionine anticodon site. Selected suppressor MTS enzymes all possess one or several mutations in the vicinity of Trp-461, a residue that is the major contributor to the stability of complexes formed with tRNAs having the cognate CAU anticodon. A genetic screening procedure was designed to isolate MTS mutants able to aminoacylate a methionine amber tRNA (CUA anticodon). Accurate aminoacylation of a tRNA by Escherichia coli methionyl-tRNA synthetase (MTS) is specified by the CAU anticodon.
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